Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 12;9:182. doi: 10.1186/s40478-021-01281-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Ser129 phosphorylation by CK2 accelerates αSyn aggregate formation. a, b WT r-αSyn or S129A r-αSyn after 0–14 days of incubation with CK2 and ATP was analyzed by SDS-PAGE followed by immunoblotting with a anti-αSyn antibody D119 and b anti-pS129-αSyn antibody ab51253. c WT r-αSyn after 0 or 15 days of incubation with CK2 and ATP was examined by TEM. Bars, 100 nm. d, e WT r-αSyn after 0 or 15 days of incubation in the presence (+) or absence (−) of CK2 or ATP was analyzed by BN-PAGE followed by immunoblotting with d anti-αSyn antibody D119 and e anti-pS129-αSyn antibody ab51253. Molecular mass markers are indicated in kDa on the left side of each panel. Arrows indicate the top of the gel. In a, b, d and e, one representative blot from three independent experiments is shown