Table 1.
List of identified peptides derived from r-αSyn incubated with CK2 and ATP for 7 days
| Query | Start | End | Observed | Mr(expt) | Mr(calc) | ppm | Score | Expect | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| WT | |||||||||
| 5284 | 44 | 58 | 802.9034 | 1603.792 | 1603.797 | − 2.97 | 65 | 3.5E−07 | K.TpKEGVVHGVATVAEK.T |
| 11012 | 46 | 80 | 1172.286 | 3513.836 | 3513.808 | 8.02 | 51 | 8.4 E−06 | K.EGVVHGVATpVAEKTKEQVTN*VGGAVVTGVTAVAQK.T |
| 8325 | 59 | 80 | 746.3851 | 2236.134 | 2236.147 | − 5.77 | 47 | 0.000021 | K.TKEQVTpNVGGAVVTGVTAVAQK.T |
| 8336 | 59 | 80 | 1120.068 | 2238.122 | 2238.115 | 3.44 | 21 | 0.0083 | K.TpKEQ*VTN*VGGAVVTGVTAVAQK.T |
| 11170 | 59 | 96 | 924.9822 | 3695.9 | 3695.914 | − 3.77 | 76 | 2.4 E−08 | K.TpKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVK.K |
| 5009 | 81 | 96 | 779.8777 | 1557.741 | 1557.744 | − 1.95 | 88 | 1.6 E−09 | K.TVEGAGSpIAAATGFVK.K |
| 12198 | 98 | 140 | 1228.245 | 4908.951 | 4908.947 | 0.96 | 20 | 0.011 | K.DQLGKN*EEGAPQEGILEDMPVDPDN*EAYEMPSpEEGYQDYEPEA |
| 12214 | 98 | 140 | 1232.002 | 4923.98 | 4923.958 | 4.6 | 34 | 0.00044 | K.DQLGKN*EEGAPQEGILEDMoPVDPDNEAYEMPSEEGYQDYpEPEA |
| 12225 | 98 | 140 | 1235.754 | 4938.985 | 4938.969 | 3.36 | 21 | 0.01 | K.DQLGKNEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA |
| 12226 | 98 | 140 | 1235.756 | 4938.994 | 4938.969 | 5.24 | 30 | 0.0011 | K.DQLGKNEEGAPQEGILEDMoPVDPDNEAYpEMoPSEEGYQDYEPEA |
| 12228 | 98 | 140 | 1236.002 | 4939.979 | 4939.953 | 5.31 | 23 | 0.0073 | K.DQLGKNEEGAPQEGILEDMoPVDPDN*EAYEMoPSEEGYQDYpEPEA |
| 12229 | 98 | 140 | 1236.003 | 4939.982 | 4939.953 | 5.91 | 26 | 0.0032 | K.DQ*LGKNEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA |
| 11769 | 103 | 140 | 1092.433 | 4365.703 | 4365.693 | 2.34 | 21 | 0.0077 | K.NEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYpEPEA |
| 11770 | 103 | 140 | 1456.242 | 4365.704 | 4365.693 | 2.61 | 18 | 0.017 | K.NEEGAPQEGILEDMPVDPDNEAYEMPSpEEGYQDYEPEA |
| 11805 | 103 | 140 | 1461.57 | 4381.688 | 4381.688 | 0.11 | 24 | 0.004 | K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA |
| 11806 | 103 | 140 | 1461.572 | 4381.693 | 4381.688 | 1.19 | 22 | 0.0069 | K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA |
| 11807 | 103 | 140 | 1461.572 | 4381.694 | 4381.688 | 1.45 | 28 | 0.0015 | K.NEEGAPQEGILEDMoPVDPDNEAYEMPSpEEGYQDYEPEA |
| 11808 | 103 | 140 | 1096.431 | 4381.696 | 4381.688 | 1.93 | 25 | 0.0034 | K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA |
| 11809 | 103 | 140 | 1461.576 | 4381.707 | 4381.688 | 4.54 | 28 | 0.0017 | K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA |
| 11851 | 103 | 140 | 1100.425 | 4397.671 | 4397.682 | − 2.69 | 31 | 0.00073 | K.NEEGAPQEGILEDMoPVDPDNEAYEMoPSpEEGYQDYEPEA |
| 11855 | 103 | 140 | 1466.906 | 4397.696 | 4397.682 | 3.13 | 31 | 0.00086 | K.NEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA |
| 11856 | 103 | 140 | 1466.908 | 4397.701 | 4397.682 | 4.29 | 24 | 0.004 | K.NEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA |
| 11870 | 103 | 140 | 1467.57 | 4399.687 | 4399.651 | 8.37 | 24 | 0.0039 | K.NEEGAPQEGILEDMoPVDPDN*EAYEMoPSpEEGYQDYEPEA |
| S129A | |||||||||
| 3243 | 11 | 23 | 691.3577 | 1380.701 | 1380.701 | − 0.32 | 28 | 0.0014 | K.AKEGVVAAAEKTpK.Q |
| 1528 | 22 | 32 | 570.2711 | 1138.528 | 1138.538 | − 9.45 | 46 | 0.000028 | K.TpKQGVAEAAGK.T |
| 4891 | 44 | 58 | 802.8981 | 1603.782 | 1603.797 | − 9.67 | 60 | 9.1 E−07 | K.TpKEGVVHGVATVAEK.T |
| 3201 | 46 | 58 | 688.3341 | 1374.654 | 1374.654 | − 0.57 | 42 | 0.00006 | K.EGVVHGVATpVAEK.T |
| 8000 | 59 | 80 | 1119.079 | 2236.144 | 2236.147 | − 1.15 | 64 | 4.2 E−07 | K.TpKEQVTNVGGAVVTGVTAVAQK.T |
| 8001 | 59 | 80 | 746.3887 | 2236.144 | 2236.147 | − 1.02 | 25 | 0.003 | K.TKEQVTNVGGAVVTGVTpAVAQK.T |
| 5372 | 81 | 97 | 843.9226 | 1685.831 | 1685.839 | − 4.98 | 52 | 6.4 E−06 | K.TVEGAGSpIAAATGFVKK.D |
p indicates phosphorylation sites; o indicates oxidation site
*Indicates deamidation site