Fig. 1.

Genomics serology to inform therapies and vaccines for arthritogenic alphaviruses. Circulating B cells binding to the ectodomain of the glycoprotein of MAYV in a convalescent donor previously exposed to CHIKV infection are single-cell cloned, and their encoded antibodies are sequenced and expressed, and tested for broadly neutralizing activity in vitro and in vivo against multiple alphavirus. Those with neutralizing properties are being used to map the recognized conserved epitope/epitopes to which they bind. This information may lead to antibody-based therapeutics and inform vaccine antigen design for the treatment or prevention of arthritogenic alphavirus infections.