Table 2.

ΔT_m of E552A/E1197A in the presence of nucleotides^[a]

		ΔTm1 (°C)[a]	ΔTm2 (°C) [b]	ΔTm3 (°C) [c]
Detergent-Solubilized	1 mM MgATP	3.6 ± 0.1		24.8 ± 0.2
	5 mM MgATP	5.2 ± 0.1		33.0 ± 0.2
	45 mM MgATP	6.0 ± 0.1		35.1 ± 0.3
Lipid-Reconstituted	1 mM MgATP	3.2 ± 0.4	3.0 ± 0.4	24.2 ± 1.7
	5 mM MgATP	5.0 ± 0.6	4.6 ± 1.1	27.4 ± 0.4
	45 mM MgATP	5.5 ± 0.4	4.9 ± 0.4	30.6 ± 0.1

^[a]The average values were calculated based on two measurements.

^[b]See Figure 6 for the numbering of the transitions.

^[c]In the detergent-solubilized Pgp, DSC transitions 1 and 2 coalesced and only one ΔT_m is reported. In Pgp proteoliposomes, the two T_ms were estimated by two-peak deconvolution (see Materials and Methods).

^[d]The reported ΔT_m3 is with respect to transition 1 in the absence of nucleotide.