FIG. 2.

A single nucleotide alteration in the spacing between the DPE and Inr reduces core promoter activity and binding of purified TFIID. (A) In vitro transcription and primer extension analysis of a series of mutant G core promoters that contain 1-, 2-, or 3-nucleotide insertions or deletions between the DPE and Inr. wt, wild type. (B) DNase I footprint analysis of G−1, G wild-type, and G+1 core promoters with purified Drosophila TFIID. Arrows indicate DNase I hypersensitive sites.