Figure 1.

CircANTXR1 was a stable circRNA with high expression in HCC. (A) GSE78520 and GSE97332 datasets exhibited the expression of circANTXR1 in HCC tumor tissues and normal tissues. (B) The information of circANTXR1 analyzed by circBase software were shown. (C) QRT-PCR was used to detect the circANTXR1 expression in HCC tumor tissues and paracancerous normal tissues. (D) The comparison of circANTXR1 expression between HCC tumor tissues (T) and paracancerous normal tissues (N) was shown. (E) CircANTXR1 expression in HCC cells (HuH-7 and HCCLM3) and THLE-2 cells was measured by qRT-PCR. (F) Kaplan-Meier analysis was used to analyze the correlation between circANTXR1 expression and overall survival rate of HCC patients. (G and H) Random primers and oligo (dT)₁₈ primers were used to explore whether circANTXR1 had poly-A tails. (I and J) RNase R assay was performed to assess the stability of circANTXR1. *P < 0.05.