Skip to main content
. 2021 Nov 9;8:1339–1353. doi: 10.2147/JHC.S317256

Figure 6.

Figure 6

XRCC5 could be targeted by miR-532-5p. (A) The binding sites and mutate sites between XRCC5 3ʹUTR and miR-532-5p were exhibited. The interaction between XRCC5 and miR-532-5p was confirmed by dual-luciferase reporter assay (B and C) and RNA pull-down assay (D). (E and F) In HuH-7 and HCCLM3 cells transfected with miR-NC or miR-532-5p mimic, XRCC5 mRNA and protein expression was measured by qRT-PCR and WB analysis. (G–J) QRT-PCR and WB analysis were used to determine XRCC5 mRNA and protein expression in HCC tumor tissues and cells. 6H showing 3 representative bands for each group in the Western blot image. (K) The correlation between XRCC5 and miR-532-5p in HCC tumor tissues was assessed using Pearson’s correlation coefficient. (L and M) The mRNA and protein expression of XRCC5 was examined by qRT-PCR and WB analysis in HuH-7 and HCCLM3 cells transfected with si-NC, si-circANTXR1#3, si-circANTXR1#3 + anti-miR-NC or si-circANTXR1#3 + anti-miR-532-5p. (N) Pearson’s correlation coefficient was performed to evaluate the correlation between XRCC5 and circANTXR1. *P < 0.05.