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Summary of the purification of extracellular protease from Bacillus sp. DEM05.
| Purification steps | Total activity (U.mL-1) | Total protein (mg.mL-1) | Specific activity (U.mg-1) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
| Culture supernatant | 3995 | 95.2 | 41.96 | 100 | 1 |
| (NH4)2SO4 (85%) precipitation | 2960 | 32.5 | 91.08 | 74 | 2.17 |
| DEAE-sepharose column | 860 | 0.8 | 1075 | 21 | 25.62 |
Each value represents the means of three experiments and the error bar indicates ±SD.
