Fig. 4.

Effects of Keguan-1 on neutrophils and alveolar macrophage in the LPS-induced ALI mouse model. (A) Representative immunofluorescent staining images for neutrophils in lung tissue at 6 h after LPS stimulation (Red, Ly6G staining; Blue, DAPI staining). (a) Control; (b) LPS; (c) LPS + 1.25 g/kg Keguan-1; (d) LPS + 2.5 g/kg Keguan-1; (e) LPS + 5.0 g/kg Keguan-1; (f) LPS + 2.5 mg/kg DXM. The upper panel is low magnification (scale bar = 100 μm) of the images, the lower panel is high magnification image (scale bar = 50 μm). (B) Statistical result of the number of Ly6G⁺-positive cells. (C) The concentration of IL-1α in BALF. (D) BALF cells were probed with anti-F4/80 and stained with annexin V and 7AAD. The number of necrotic cells was determined by using flow cytometry. The data are presented as the mean ± SEM. ^#P < 0.05 and ^##P < 0.01 vs. control; ^∗P < 0.05 and ^∗∗P < 0.01 vs. LPS group.