Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 13;13:204. doi: 10.1186/s13148-021-01192-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Southern blot analysis showing the differential DNA methylation status of the NOTCH2NLC region. a Physical map of the NOTCH2NLC region with the NheI, MspI/HpaII restriction enzyme sites and Southern blot probe. Chromosome positions are based on hg38 assembly. Black, green, blue and orange rectangles represent (GGC)n/(GGCGGA)n, CpG island, SINE and Southern blot probe, respectively. b Genomic DNA extracted from blood samples (F2 family) and LCLs (F3 and F4 families) were digested by the restriction enzyme indicated in the figure panel. N + M: NheI and MspI; N + H: NheI and HpaII; N: NheI. Note that expanded alleles in the father’s sample are resistant to restriction digestion by the methylation-sensitive endonuclease HpaII (arrow). Asterisk: cross hybridization to the NOTCH2NLC homologous genes, NOTCH2, NOTCH2NLA, NOTCH2NLB and NOTCH2NLR. Note that two prominent signals (arrows) were detected in the blood sample from the F2-father, but only one signal (arrow) was detected in LCLs of the F3-father and F4-father, indicating somatic mosaicism