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. 2021 Nov 14;21:1224. doi: 10.1186/s12885-021-08967-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — CRBP-1 contributes to the cell growth in vitro and tumorigenicity in vivo of HCC. (A) Western-blot analysis of the expression of endogenous CRBP-1 in HCC cell lines. (B) Cell proliferation analysis of CRBP-1 overexpressed and control PLC/PRF/5 and Huh7 cells. All data shown represent the mean ± SD from three independent experiments. (C) Colony formation analysis of CRBP-1 overexpressed and control PLC/PRF/5 and Huh7 cells. All data shown represent the mean ± SD from three independent experiments. (D) Western-blot analysis of the expression of Ki-67 in CRBP-1 overexpressed and control PLC/PRF/5 and Huh7 cells. (E) The tumor formation rate of CRBP-1 overexpressed and control PLC/PRF/5 cells inoculated in Balb/c nude mice. (F) The tumor growth curve was shown in CRBP-1 overexpressed PLC/PRF/5 cells and control ones. All data shown represent the mean ± SD from indicated numbers of xenograft tumor. (G) The photograph of xenograft tumors respectively derived from CRBP-1 overexpressed and control PLC/PRF/5 cells inoculated in Balb/c nude mice