FIG. 3.

Analysis of binding of HuD deletion mutants to AU-3 RNA. (A to F) Increasing concentrations of HuD or deletion mutants were equilibrated with AU-3 RNA and analyzed by gel shift assays. The protein concentration is given below each gel. ∗ and P-, complex and probe (free RNA), respectively. (G) The gel shift data were quantitated and plotted as in Fig. 1. The graph includes data from RRM1+2 and dNterm binding reactions (gels not shown) and HuD binding reactions (Fig. 1) for comparison.