TABLE 1.
In vitro-transcribed AU-rich RNA targetsa
| Target name | Sequence (length in nucleotides)b |
|---|---|
| AU-1 (nonamer) | gggagacccaagcUUAUUUAUUgcaggucg (30) |
| AU-3 | gggagacccaagcUUAUUUAUUUAUUUAUUgcaggucg (38) |
| (AUUU)2A | gggagacccaagcuugcAUUUAUUUAccugcaggucg (37) |
| MUT | gggagacccaagcUUAUCUAUCUAUCUAUUgcaggucg (38) |
| AU-2 | gggagacccaagcUUAUUUAUUUAUUgcaggucg (34) |
| AU-1+ | gggagacccaagcUUAUUUAUUUgcaggucg (31) |
a
5′ (left) to 3′ (right) direction. Targets shown were used for gel shift analyses. The AU tract is uppercase, flanking sequences are lowercase. For attachment to BIACORE sensor chips, RNAs carried the 3′ extension gacucuagaggauccccgg.
b
The nonamer motif UUAUUUAUU is indicated by an underline or bold type. In two adjacent nonamers the abutting U's were not marked for reasons of clarity. Two adjacent nonamers yield three overlapping nonamer sequences.