TABLE 2.

Kinetic values for complexes of HuD and mutants with AU-3 RNA

Fig. 4 panel	Protein	ka (M−1 s−1)	kd (s−1)	Kd (nM)
A	HuD	(4.21 ± 0.12) × 106	(3.05 ± 0.047) × 10−3	0.7 ± 0.02
B	RRM1+2+h	(15.0 ± 0.13) × 106	(43 ± 0.5) × 10−3	2.9 ± 0.04
C	RRM1+2	(34.9 ± 0.49) × 106	(190 ± 3.4) × 10−3	5.4 ± 0.12
D	RRM1+h+3	(11.4 ± 0.07) × 106	(108 ± 1) × 10−3	9.4 ± 0.1
E	RRM2+h+3	(0.20 ± 0.02) × 106	(310 ± 15) × 10−3	1,550 ± 17