Table 2.
The SARS-CoV-2 mutational landscape at the peak of the first wave of the pandemic.a
| Protein | Site | Entropy Δb | Str | Location | Region | IUPred2 | Anchor2 |
|---|---|---|---|---|---|---|---|
| M | 175 | –0.136 | loop | surface | ordered | 0.2167 | 0.2647 |
| N | 13 | 0.050 | loop | surface | disordered | 0.8781 | 0.9749 |
| 193 | –0.073 | loop | surface | disordered | 0.7912 | 0.4214 | |
| 197 | –0.064 | loop | surface | disordered | 0.8158 | 0.4259 | |
| 203 | 0.157 | loop | buried | disordered | 0.7573 | 0.5969 | |
| 204 | 0.143 | loop | buried | disordered | 0.7605 | 0.621 | |
| S | 614 | –0.220 | loop | surface | ordered | 0.1266 | 0.3609 |
| NSP1 | 75 | –0.016 | loop | surface | ordered | 0.3184 | 0.4256 |
| NSP2 | 85 | 0.055 | loop | surface | ordered | 0.0771 | 0.261 |
| 212 | –0.120 | loop | surface | ordered | 0.2748 | 0.2408 | |
| 559 | –0.151 | loop | surface | ordered | 0.1921 | 0.345 | |
| 585 | –0.163 | loop | surface | ordered | 0.4245 | 0.4187 | |
| NSP3 | 58 | –0.101 | helix | surface | ordered | 0.0414 | 0.2217 |
| 153 | –0.020 | loop | surface | disordered | 0.8074 | 0.9536 | |
| NSP4 | 308 | –0.071 | loop | surface | TM | 0.0004 | 0.0003 |
| NSP5 | 15 | 0.114 | helix | surface | ordered | 0.0813 | 0.0843 |
| NSP6 | 37 | –0.121 | loop | surface | ordered | 0.0003 | 0 |
| NSP12 | 323 | –0.222 | helix | surface | ordered | 0.0137 | 0.0299 |
| NSP13 | 504 | –0.226 | loop | surface | ordered | 0.115 | 0.4329 |
| 541 | –0.231 | loop | surface | ordered | 0.3053 | 0.3068 | |
| 3a | 13 | 0.082 | loop | surface | ordered | 0.0587 | 0.029 |
| 57 | 0.066 | helix | pore | TM | 0.001 | 0.0014 | |
| 196 | –0.072 | loop | surface | ordered | 0.115 | 0.2522 | |
| 251 | –0.217 | loop | surface | disordered | 0.4703 | 0.4235 | |
| 8 | 24 | 0.105 | helix | surface | ordered | 0.0395 | 0.004 |
| 62 | –0.015 | loop | surface | ordered | 0.1635 | 0.0205 | |
| 84 | –0.259 | loop | surface | ordered | 0.0405 | 0.0098 |
An examination of relative entropy delta values and the structure (Str: loop, helix, strand), location (surface, buried, pore) and region [ordered, disordered, transmembrane (TM)] involving mutation sites are provided together with IUPred2 scores of intrinsic disorder and Anchor2 estimates of binding propensity. IUPred2 and Anchor2 scores above 0.5 support significant intrinsic disorder and binding energy of that amino acid site, respectively. Data from Tomaszewski et al. (2020).
Negative values of relative entropic delta imply entropy reversals that result in increased fitness and trends towards fixation. Positive values imply entropy expansion fostering trends towards site diversification. Rows in bold are sites that have been confirmed as positively selected and exhibiting population level expansions as of March 2021 (from table 2, Singh et al., 2021).