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. 2021 Nov 10;13:8435–8443. doi: 10.2147/CMAR.S256091

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© 2021 Zhang et al.

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Hsa_circ_0055478 (circPTCD3) is upregulated in breast cancer specimens and cell lines. (A) Hsa_circ_0055478 also named as circPTCD3 was back-spliced by exons 3–9 of PTCD3 gene as the black arrow showed. The existence of circPTCD3 were confirmed by sanger sequencing, the red arrow shows the head-to-tail splicing junction site of circPTCD3. (B) After treatment with Actinomycin D, the amount of circular and linear PTCD3 from breast cancer cells were evaluated using qPCR. (C) FISH assay with specific probe was performed to detect the expression and location of circPTCD3. (D) After RNase R treatment, the expression of linear and circular PTCD3 were detected using qPCR. Expression level of circPTCD3 was evaluated by qPCR in breast cancer tissues (E) and cell lines (F) compare to the adjacent normal tissues and MCF-10A cell. n=6, *P< 0.05, compared with the normal, mock, MCF-10A group correspondingly, **P< 0.01, compared with the normal.