Table 1.
The reported O-glycosites and O-glycopeptides in S protein
| Expression system | Glycosites and glycopeptides | Description | Refs. |
|---|---|---|---|
| T323, S325 | Low occupancy (<1%) | 63 | |
| T323, S325 | Core-1 and Core-2 O-glycans at T323 | 70 | |
| T678 | Core-1 and Core-2 O-glycans, 13% occupancy | 332 | |
| O-glycopeptides: CDIPIGAGICASYQTQTNSPR, TQLPPAYTNSFTR, YFKNHTSPDVD, VQPTESIVR, VYSTGSNVFQTR, VPVAIHADQLTPTWR, STECSNLLLQYGSFCTQLNR, LPDDFTGCVIAWNSNNLD, YSVLYNSASFSTFK | The glycopeptides are identified, but the glycosites and the glycans are not characterized | 332 | |
| Human cells | T22, T29, S31, T33, T124, T284, T286, S297, T299, T302, S305, T307, T315, S316, T323, S325, T572, T573, T581, T630, T632, S637, T638, S640, T645, S659, S673, T676, T678, S680 | Mainly modified with Mucin-type O-glycans | 339 |
| T323, S325 | Core-1 and Core-2 O-glycans at T323 | 328 | |
| T63, T73, T76, T114, T167, T236, T478, T618, T676, T678, S803, T1076, T1077, S1097 | Low overall occupancy | 336 | |
| T73, S221, T284, T323, S325, T547, T618, T630, T632, S673, T716, S803, S810, S813, S975, S1123, T1136, S1175 | Mainly modified with HexNAc(2)Hex(3) | 338 | |
| T323 | Core-1 and Core-2 O-glycans at T323 | 340 | |
| Human cells | T22, T29, S31, T33, S50, T51, S71, T73, S94, T95, S98, T108, T109, S247, T250, S254, S255, S256, T259, T286, S297, T299, T315, S316, T323, S325, T333, S349, S359, S366, S371, S375, T376, T430, T470, S477, T478, S494, T500, T523, T547, T553, S555, T581, S591, T599, T630, T632, S637, T638, S640, T645, S673, T676, T678, S680 | GalNAc and GalGalNAc mucin-type O-glycans at T323 and T325 | 342 |
| Insect cells | T333, S438, S443, S514, T523, S366, S371, S373, S375, T376 | The glycan types are not determined | 333 |
| T22, T29, S31, T33, S50, T51, S71, T73, S94, T95, S98, T108, T109, S247, T250, S254, S255, S256, T259, T286, S297, T299, T315, S316, T323, S325, T333, S349, S359, S366, S371, S375, T376, T430, T470, S477, T478, S494, T500, T523, T547, T553, S555, T572, T573, T581, S591, T599, T630, T632, S637, T638, S640, T645, S673, T676, T678, S680 | T22, T29, S31, T33, S94, T95, T323 and T325 are the most abundant O-glycosites | 342 | |
| T22, T29, S31, S94, T95, T114, S116, T124, T284, T286, S297, T299, T323, S325, T333, T345, S477, T572, T573, S659, S673, T676, T678, T732, T791, S803, S810, S813, T912, S939, S940, T941, T1066, T1076, T1077, S1097, T1100, T1105, T1160, S1161, S1170, S1175, S1196 | Mucin-type O-glycans | 339 | |
| Insect cells | T63, S71, T73, T76, S94, T95, T124, S151, T315, T323, T333, T345, T415, T523, T678, S803, T1076, T1077, S1097, T1100 | Low overall occupancy | 336 |
| From SARS-CoV-2 virions | T22, S60, T124, S151, T236, S305, T307, T323, T604/S605, T618, S659, T696, T724, T1076, T1077, S1097, T1100 | Occurred together with N-glycosites in N-sequon-associated positions | 80 |
| Chinese hamster ovary cells | T323, S325 | Core-1 O-glycans at T323 | 341 |
| Not available | S673, T678, S686 | Predicted O-glycosites | 337 |
| Not available | T323, S325 | Core-1 and Core-2 O-glycans | 62 |