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. 2021 Nov 1;11:715677. doi: 10.3389/fonc.2021.715677

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Copyright © 2021 Dezfuli, Alipoor, Dalil Roofchayee, Seyfi, Salimi, Adcock and Mortaz

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Treg and CD3/CTLA-4 cells in blood of NSCLC patients. (A) Upper panel: Gating strategy for Treg cells frequency; analysis of a blood sample for CD25, as well as FoxP3 expression in the CD4+ lymphocyte gate. (A) Lower panel: Gating strategy for CD3+CTLA4+ cells percentage. Analysis of a blood sample for CTLA4 expression in the CD3+ lymphocyte gate. (B) Flow cytometry analysis of frequencies Treg and CD3/CTLA-4 in blood cells stained with related antibodies of NSCLC and healthy control subjects, which was calculated statistically and plotted to the graph (P ≤ 0.0001). The dots indicate the means, and the error bars indicate the standard deviations of all patients and healthy control measurements.