Table 5.
Pathway enrichment of gene expression correlating positively with glyoxalase 1 expression in tumor cell lines of the CCLE.
| Pathway | Count | Fold enrichment | FDR | Genes |
|---|---|---|---|---|
| Spliceosome | 20 | 7.0 | 3.4 × 10−9 | CDC5L, HNRNPA1, HNRNPA3, HNRNPC, HNRNPK, LSM2, LSM3, LSM5, MAGOH, MAGOHB, PPIL1, PRPF4, RBMXL1, SNRNP40, SNRPB, SNRPC, SNRPD1, SNRPE, SNRPG, SRSF3 |
| RNA transport | 22 | 6.0 | 3.4 × 10−9 | DDX20, EIF1AX, EIF2S1, EIF4E, GEMIN5, GEMIN6, KPNB1, MAGOH, MAGOHB, NDC1, NUP35, NUP37, NUP43, NUP54, NUP107, NUP153, NUP155, RPP40, SUMO1, SUMO2, XPO1, XPO5 |
| Cell cycle | 19 | 7.2 | 3.4 × 10−9 | CCNB1, CCNB2, CCNE2, CDC25C, CDC27, CDC7, CDK1, DBF4, HDAC2, MAD2L1, MCM3, ORC2, ORC3, ORC4, PTTG1, SKP1, SKP2, PRIM2, TTK |
| DNA replication | 10 | 12.7 | 1.2 × 10−6 | DNA2, MCM3, POLA1, POLE2, PRIM2, RFC2, RFC3, RFC4, RFC5, RPA2 |
| Proteasome | 6 | 6.4 | 0.037 | PSMD12, PSMA4, PSMD14, PSMB3, PSMC1, PSMB1 |
Pathway enrichment analysis was performed for 340 genes correlating positively with Glo1 RNA copy number in the CCLE database with r2 ≥ 0.1 using the KEGG database; data from 1,010 tumor cell lines for 10,758 genes, applying a Bonferroni correction of 10,758 ( Supplementary Table S12 ). Statistical threshold criterion was FDR <0.05. Genes unrecognized in KEGG were pseudogene (2), antisense RNA (7), microRNA (2), long noncoding RNA (4), other intronic RNA (1), and uncharacterized proteins (1). Genes highlighted in bold are those encoding for proteins that were decreased in the early-stage proteomic response to MG-induced cytotoxicity.