TABLE 1.
List of proteins involved in the GPI biosynthesis pathway.
| Enzyme type |
Homo sapiens
|
Saccharomyces cerevisiae
|
Trypanosoma brucei
|
Leishmania mexicana
|
Trypanosoma cruzi
|
|||||
| Name | UniProtKB | Name | UniProtKB | TriTrypDB | e-value | TriTrypDB | e-value | TriTrypDB | e-value | |
| GPI-GlcNAc transferase | PIG-A | P37287 | GPI3 | P32363 | Tb927.2.1780 | 1E-150 | LmxM.32.1670 | 2E-146 | TcCLB.510259.18 | 3E-151 |
| PIG-C | Q92535 | GPI2 | P46961 | Tb927.10.6140 | 1E-30 | LmxM.36.1710 | 9E-30 | TcCLB.507639.100 | 5E-38 | |
| PIG-H | Q14442 | GPI15 | P53961 | |||||||
| PIG-P | P57054 | GPI19 | Q04082 | Tb927.10.10110 | 4E-12 | LmxM.36.4750 | 6E-11 | TcCLB.508307.100 | 4E-12 | |
| PIG-Q | Q9BRB3 | GPI1 | P53306 | Tb927.3.4570 | 8E-13 | LmxM.08_29.2030 | 3E-14 | present in non-reference strains | ||
| PIG-Y | Q3MUY2 | ERI1 | P62651 | |||||||
| DPM2 | O94777 | Tb927.9.6440 | 1E-3 | LmxM.15.0815 | 3E-6 | TcCLB.506579.119 | 6E-4 | |||
| TcCLB.510043.29 | 6E-4 | |||||||||
| GlcNAc-PI de-N-acetylase | PIG-L | Q9Y2B2 | GPI12 | P23797 | Tb927.11.12080 | 3E-36 | LmxM.09.0040 | 7E-37 | TcCLB.504005.20 | 8E-33 |
| Inositol acyltransferase | PIG-W | Q7Z7B1 | GWT1 | P47026 | ||||||
| Mannosyl- transferase I | PIG-M | Q9H3S5 | GPI14 | P47088 | Tb927.6.3300 | 3E-64 | LmxM.29.2030 | 9E-62 | TcCLB.503909.100 | 1E-64 |
| PIG-X | Q8TBF5 | PBN1 | P25580 | |||||||
| Mannosyl- transferase II | PIG-V | Q9NUD9 | GPI18 | P38211 | Tb927.10.13160 | 1E-9 | LmxM.18.0960 | 3E-8 | TcCLB.506359.30 | 2E-11 |
| EtN-P transferase I | PIG-N | O95427 | MCD4 | P36051 | Tb927.11.5070 | 1E-4 | LmxM.24.0340 | 2.5E-2 | TcCLB.507667.11 | 9.9E-2 |
| Mannosyl- transferase III | PIG-B | Q92521 | GPI10 | P30777 | Tb927.10.5560 | 5E-47 | LmxM.36.1200 | 9E-25 | TcCLB.503527.40 | 3E-43 |
| EtN-P transferase III | PIG-O | Q8TEQ8 | GPI13 | Q07830 | Tb927.11.5070 | 1E-44 | LmxM.24.0340 | 4E-41 | TcCLB.507667.11 | 6E-37 |
| PIG-F | Q07326 | GPI11 | Q06636 | |||||||
| EtN-P transferase II | PIG-G | Q5H8A4 | GPI7 | P40367 | Tb927.11.5070 | 4E-28 | LmxM.24.0340 | 4E-33 | TcCLB.507667.11 | 3E-28 |
| PIG-F | Q07326 | GPI11 | Q06636 | |||||||
| Mannosyl-transferase IV | PIG-Z | Q86VD9 | SMP3 | Q04174 | ||||||
| Lyso-GPI acyltransferase | PGAP2 | Q9UHJ9 | CWH43 | P25618 | LmxM.27.1770 | 1.7E-2 | TcCLB.504153.120 | 2E-11 | ||
| GUP1 | P53154 | Tb927.10.15910 | 2E-42 | LmxM.19.1347 | 5E-50 | TcCLB.511355.40 | 1E-51 | |||
| GPI transamidase | PIG-K | Q92643 | GPI8 | P49018 | Tb927.10.13860 | 8E-63 | LmxM.18.0360 | 3E-57 | present in non-reference strains | |
| GAA1 | O43292 | GAA1 | P39012 | Tb927.10.210 | 7.4E-2 | |||||
| PIG-S | Q96S52 | GPI17 | Q04080 | |||||||
| PIG-T | Q969N2 | GPI16 | P38875 | * | ||||||
| PIG-U | Q9H490 | GAB1 | P41733 | |||||||
| Tb927.11.15760 | LmxM.31.2560 | TcCLB.511545.190 | ||||||||
| Tb927.10.5080 | LmxM.36.0650 | TcCLB.510293.79 | ||||||||
All known proteins involved in the biosynthesis of mammalian (e.g., Homo sapiens) and yeast (Saccharomyces cerevisiae) GPIs were BLASTed for homology in Trypanosoma brucei, Trypanosoma cruzi, and Leishmania mexicana. The BLAST was performed with the annotated protein sequences of H. sapiens acquired from UniProtKB against all trypanosomatid protein sequences currently annotated in TriTrypDB. In the case of GUP1, which is not present in the mammalian biosynthesis pathway, the protein sequence from Saccharomyces cerevisiae was used. All Gene-IDs and respective e-values given refer to the current reference strains. *Our BLAST did not find a homolog for the GPI transamidase subunit PIG-T. However, a previous study found an ortholog of mammalian PIG-T and yeast GPI16 in T. brucei (Nagamune et al., 2003).