Table 3.
Details of Statistical Tests
| Figure 1 | ||||||
| A | Two-way ANOVA | f(6, 52) = 2.766; P = .0208 | J | Unpaired t test | P < .0001 | |
| C | One-way ANOVA Tukey post-test |
f(3, 46) = 80.56; P < .0001 P < .0001 |
K | Unpaired t test | P = .7113 | |
| D | One-way ANOVA Tukey post-test |
f(3, 47) = 57.41; P < .0001; P < .0001 |
L | Unpaired t test | P = .5447 | |
| F | Unpaired t test | P = .0338 | M | Unpaired t test | P = .4881 | |
| H | One-way ANOVA Tukey post-test |
f(2, 390) = 5.936; P = .0029; ZT12 P = .0030 |
O | Unpaired t test | P = .3793 | |
| I | Unpaired t test | P = .1036 | ||||
| Figure 2 | ||||||
| B | Unpaired t test | P = .0012 | J | Unpaired t test | P = .0013 | |
| D | Unpaired t test | P = .0166 | K | Unpaired t test | P < .0001 | |
| F | Two-way ANOVA Tukey post-test |
f(2, 218) = 3.386; P = .0352 ZT4 P = .0246 |
L | Unpaired t test | P = .0123 | |
| G | Unpaired t test | P = .031 | N | Unpaired t test | P = .0005 | |
| I | Unpaired t test | P = .1409 | P | Unpaired t test | P < .0001 | |
| Panel | Test | Value | |||
|---|---|---|---|---|---|
| Figure 3 | |||||
| C | MetaCycle2d | Nr1d1 (P = 4.9-08; q = 0.00039), Cry1 (P = 3.8-08; q = 0.00039), Bmp3 (P = .00012; q = 0.057), Ephb3 (P = 8.0-06; q = 0.0084), Kit (P = .00016; q = 0.067), Tead4 (P = 1.09-05; q = 0.0098) | |||
| D | Two-way ANOVA | Nr1d1 (f(12, 37) = 5.993; P < .0001), Cry1 (f(1, 37) = 413.1; P < .0001), Tead4 (f(12, 37) = 15.36; P < .0001) | |||
| Figure 4 | |||||
| D | MetaCycle2d | Golga (P = 1.20-06; q = 0.015), Dolk (P = 2.83-06; q = 0.015), RNF128 (P = 5.81-06; q = 0.015), Mlxipl (P = 4.26-07; q = 0.0053), Msmo1 (P = 1.04-06; q = 0.0053), Fam76b (P = 1.44-06; q = 0.0055) |
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| E | Two-way ANOVA | Golga (f(12,26) = 8.393; P < .0001), Dolk (f(12,26) = 3.557; P = .0033) RNF128 (f12, 25) = 3.284; P = .0058), Mlxipl (f(12,15) = 1.220; P = .3623), Msmo1(f12, 13) = 2.051; P = .1067), Fam76b (f(12, 26) = 6.062; P < .0001) |
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| Panel | Test | Value | Panel | Test | Value | |||
|---|---|---|---|---|---|---|---|---|
| Figure 5 | ||||||||
| A | Gene-wise 1-way ANOVA (4 genotypes) with BH correction Gene-wise Tukey post-test (2 genotypes) with BH correction |
f(3,84) > 2.75; P < .047; q < 0.05; P < .00357; q < 0.05 | H | Unpaired t test | P = .0075 | |||
| B | Gene-wise 1-way ANOVA (4 genotypes) with BH correction Gene-wise Tukey post-test (2 genotypes) with BH correction |
f(3,84) > 2.75; P < .047; q < 0.05; P < .02815; q < 0.05 | K | Unpaired t test | P = .0444 | |||
| C | GO analysis: DAVID 6.8 |
P < .0002; Benjamini < 0.05 Gene set > 9 |
L | One-way ANOVA Tukey post-test Apcmin; Bmal1-/-DMSO vs Yap-i |
f(3, 10) = 19.55; P = .0002; P = .0068 | |||
| D | ES NES Nominal P value FDR q-value FWER P value |
ES, 0.44; NES, 2.083; P = .000; q =0.000; P = .000 | M. | One-way ANOVA Tukey post-test: DMSO vs DMSO Apcmin; Bmal1-/-DMSO vs YAP |
f(2,43) = 4.69; P = .0144; P = .0272; P = .0044 | |||
| G | Unpaired t test | P = .0004 | ||||||
| Figure 6 | ||||||||
| A | One-way ANOVA Tukey post-test |
f(3,8) = 11.48; P = .0026; P = .0029 | D | One-way ANOVA Tukey post-test |
f(3, 16) = 8.420; P = .0014; P = .0217 | |||
| B | Unpaired t test | 0.9172 | F | One-way ANOVA Tukey post-test |
f(3, 65) = 6.824; P = .005; P = .0182 | |||
| C | Unpaired t test | P = .027 | ||||||
| Figure 7 | ||||||||
| B | Unpaired t test | P = .0186 | G | One-way ANOVA Sidak post-test |
f(3, 36) = 9.241; P = .0001 P = .0096 |
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| E | Unpaired t test | P = .0009 | I | One-way ANOVA Sidak post-test |
f(3, 36) = 9.422; P < .0001 P = .0064 |
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| D | Unpaired t test | P = .027 | ||||||
| Figure 8 | ||||||||
| A | Two-way ANOVA | f(5, 168) = 12.01; P < .0001 | H | Two-way ANOVA Tukey post-test |
f(2, 116) = 351.9; P < .0001 ZT4 P < .0001 ZT12 P < .0001 |
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| C | Two-way ANOVA | f(5, 168) = 6.49; P < .0001 | J | Mixed-effect analysis Sidak post-test |
f(2, 27) = 127.4; P < .0001 ZT12 < 0.0001 |
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| E | Two-way ANOVA | f(1, 168) = 25.50; P < .0001 | ||||||
| Panel | Test | Value |
|---|---|---|
| Figure 9 | ||
| A | Two-way ANOVA | f(5, 168) = 2.50; P = .033 |
| C | Two-way ANOVA | f(5, 168) = 9.24; P = .0027 |
| E | Two-way ANOVA | f(1, 168) = 8.947; P = .0032 |
| G | Two-way ANOVA | f(1, 168) = 71.98; P < .0001 |
| I | Two-way ANOVA | f(1, 168) = 41.51; P < .0001 |
| K | Two-way ANOVA | F(5, 168) = 2.746; P = .02 |
| Panel | Test | Value | Panel | Test | Value |
|---|---|---|---|---|---|
| Figure 10 | |||||
| B | One-way ANOVA Tukey post-test |
f(3, 20) = 20.58; P < .0001 P = .0027 |
G | Unpaired t test | P = .0341 |
| C | One-way ANOVA Tukey post-test |
f(3, 20) = 28.71; P < .0001 P = .0014 |
I | Unpaired t test | P = .0447 |
| E | Unpaired t test | P = .0023 | |||
ANOVA, analysis of variance; DMSO, dimethyl sulfoxide; ES, enrichment score; FDR, False Discovery Rate; FWER, Family-Wise Error Rate; NES, normalized enrichment score; Yap-i, Yap Inhibitor; ZT, Zeitgeber Time.