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. 2021 Nov 1;9:749129. doi: 10.3389/fcell.2021.749129

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Copyright © 2021 Gui, Zhao, Sun, Shao, Huang, Zhao and Kuang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ATG5 knockdown inhibited autophagy of GC cells promoted by overexpression of LncFEZF1-AS1. (A) CCK8 assay on the proliferation of SGC-7901 cells with or without the treatment of FEZF1-AS1, FEZF1-AS1 + ATG5 siRNA or their control. (B) The proliferation of SGC-7901 with or without the treatment of FEZF1-AS1 siRNA, FEZF1-AS1 siRNA + ATG5 or their control group analyzed by CCK8 assay. (C) Representative images of LC3 puncta formation in SGC-7901 observed via IF staining, green: LC3B, bule: DAPI, scar bar: 20 mM. The results represented the mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.