Figure 4.

Elevated B1 cells and oxLDL-IgM levels in agonistic PD-1 treated mice. Total CD19⁺ B cells, B1 cells (CD19⁺B220^lo) and regulatory B cells (CD19⁺CD5⁺CD1d^hi) were measured by flow cytometry in blood (A). Serum Cu-oxLDL IgM (relative light units (RLU)/100 ms), total IgM (μg/ml), Cu-oxLDL IgM/total IgM ratio, and Cu-oxLDL IgG (RLU/100ms) were measured by ELISA (B). Mean ± SEM are shown. Statistics was performed using unpaired T-test. P ≤ 0.05 are considered significant. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.