FIGURE 2.

Methyltransferase-like 3 impacts m⁶A methylation and EPC-mediated angiogenesis. (A) Total m⁶A RNA methylation levels were quantified in EPCs following knockdown or overexpression. (B) Endothelial progenitor cell proliferation was measured via CCK-8 assay. METTL3 overexpression enhanced cellular proliferation, while silencing impairs such proliferation. (C–F) In vitro EPC angiogenesis was measured through Transwell (C), Wound healing (D), and Tube formation assays (E). Scale bars: 100 and 500 μm. (F) METTL3 affects ex vivo angiogenesis. CAM assays revealed that METTL3 overexpression significantly increased vascular density, while METTL3 inhibition had the opposite effect. NC-OE and OE-METTL3 groups or NC-shRNA and METTL3-shRNA groups were compared via Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001. All cell experiments were conducted in triplicates using three individual cell lines.