Fig. 2.

scRNA-seq to classify cell types in orthotopic tumors induced with Panc02 and Panc02-H7 cells. Fresh single cells were prepared in two different tumors formed with Panc02 and Panc02-H7 cells. Total 4470 viable cells in Panc02 cell-formed tumors and 4586 viable cells in Panc02-H7 cell-formed tumors underwent scRNA-seq. (A) Schematic depicting scRNA-seq experiment and analysis. (B) Unsupervised clustering of viable cells from two different tumors, represented as a t-SNE plot. Different cell type clusters are color coded. (C) Bubble blot showing selected cell type-specific markers across all cell clusters. The size of each circle reflects the percentage of 16 cell clusters expressing particular markers, and the intensity of color reflects the level of average expression. Red circles and blue circles, respectively represent the cells from Panc02-H7- and Panc02-formed tumors. (D) The frequency of final eight major cell clusters. Based on the number of each cell cluster, the frequencies of final eight cell clusters were counted in two types of tumors formed with Panc02-H7 and Panc02 tumors. (E) Heat map showing differentially expressed genes in three cancer cell clusters in Panc02 and Panc02-H7 induced PaC tumors. Only genes were selected if their expressions are different in any pairwise comparison between two tumors or different clusters. Each row represents a gene. Color scheme represents Z-score distribution from −2 (blue) to 2 (red).