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. 2021 Nov 9;15(1):101262. doi: 10.1016/j.tranon.2021.101262

Fig. 4.

Fig. 4

More exhausted T cells in tumors induced with Panc02 than Panc02-H7 cells. tSNE plot shows the distribution of activated (A) and exhausted (B) T cells with the indicated makers in total four clusters of T cells in Panc02 and Panc02-H7 induced PaC tumors. The cells containing more than 1 count of particular gene were selected using the loupe software. The absolute cells in two types of tumors are shown in Histogram. (C) The frequency of tumor-infiltrating CD8+ T cells positive for PD-1 and CD69 in two different tumors. Flow cytometry is used to detect the CD8+ T cells positive for PD-1 and CD69 in total viable cells.  The accumulated results were shown. n = 5, ***p < 0.001. (D) Representative IHC staining of PD-1 and GZMB in tumors formed with Panc02 and Panc02-H7 cells. PD-1 and GZMB in the formalin-fixed paraffin-embedded (FFPE) tumor sections were stained with the relevant antibodies. The semi-quantitative assay is used to show the tumor infiltrating PD-1+ and GZMB+ cells per mm2 tumor field. Scale bars, 50 µm, n = 5, ***p < 0.001.