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. 2021 Nov 9;15(1):101262. doi: 10.1016/j.tranon.2021.101262

Fig. 5.

Fig. 5

Characterization of tumor-infiltrating macrophages in two types of tumors. (A) tSNE plot shows TAM positive for Adgre1 (F4/80) in the tumors induced with Panc02 and Panc02-H7 cells. (B) The frequency of total, M1- and M2-type TAMs in two different tumors. In terms of expression of the indicated genes in scRNA-seq, the percentage of total TAM positive for Adgre1 (F4/80) in the entire tumor infiltrated cells (left panel), M1-type TAM positive for CD86 (middle panel) and M2 TAM positive for CD206 (right panel) in TAM population were defined. CD86+and CD206+TAM were selected when the cells contain more than 1 count of gene CD86 or CD206 using the loupe software. (C) Flow cytometric assay of the frequency of total, M1-, and M2-type of TAMs. We detect a significant increase in the proportion of total TAM (F4/80+CD11b+) (left panel), no difference in the percentage of M1 phenotype (F4/80+CD11b+CD86+) (middle panel), and significant decrease in the proportion of M2-type TAM (F4/80+CD11b+CD206+) (right panel) in Panc02-H7-formed tumors in comparison to that in Panc02-formed tumors, n = 5, *** p < 0.001. (D) IHC staining of F4/80+, CD86+ (M1) and CD206+ (M2) cells in two different tumors. The semi-quantitative assay defines tumor infiltrating F4/80+ cells, the ratio of CD86+ and F4/80+cells, the ratio of CD206+and F4/80+cells per mm2 field. Scale bars, 50 µm. n = 5, *** p < 0.001.