Fig. 6.

TAMs with antigen-presenting function in Panc02-H7 tumors, but immunosuppressive function in Panc02 tumors. (A) Heat map showing difference in the gene expressions in TAM1 and TAM2 cell clusters from Panc02 and Panc02-H7 induced PaC tumors. Each row represents one gene. Only genes are selected if their expressions are different in any pairwise comparison between two tumors or two different clusters. Color scheme represents Z-score distribution from −1.5 (blue) to 1.5 (red). (B, C) Scatter plot showing the genes that exhibit dramatic difference in TAM1 (B) and TAM2 (C) between two tumors. The genes expressed in TAM1 and TAM2 with dramatic difference in the two different tumors are highlighted with blue (Panc02-H7) and red (Panc02) circles. More increased expression of antigen presenting genes and decreased expression of immunosuppressive genes were detected in TAM1 and TAM2 in Panc02-H7-formed tumors compared to Panc02-formed tumors. (D) Detection of MHC II gene H2-Ab1 and MHC II associated gene CD74 mRNA expression in two different tumors. qPCR is used to measure the mRNA expression of H2-Ab1 and CD74 in Panc02- and Panc02-H7-formed tumors. n = 7, * p < 0.05 and ** p < 0.01. (E) Detection of MHC II protein expression in two different tumors. Western blotting is used to measure the protein expression of MHC II in Panc02- and Panc02-H7-formed tumors. (F) Significantly enriched gene sets from GSEA analysis against the Hallmarks MSigDB collection for tumor cell RNA-seq data using differentiated expressed genes from every cluster. TAM1 clusters are selected with top 10 enriched gene set; TAM 2 clusters are selected with all enriched gene set.