Figure 1.

Overview of the experimental design to investigate tissue of primary colorectal carcinoma and patient-matched liver metastasis.

(A) Primary colorectal carcinoma tissue (blue) and patient-matched liver metastasis tissue (yellow) were collected from seven patients. Following surgical removal, the tissue was conserved using formalin fixation and paraffin-embedding (FFPE). (B) Small, 10 µm thick tissue slices were deparaffinized. For each sample, the tumorous tissue was macro dissected and transferred to fresh microcentrifugation tubes. Protein extraction and heat-induced antigen retrieval (HIAR) in combination with a direct trypsinization (DTR) protocol was applied. Following peptide clean-up, the samples were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Differential expression analysis was performed, and individual target proteins were used for Immunohistochemistry (IHC) and cell proliferation assays.