Table 1.
Batch‐to‐batch analyses of the NF
| Parameter | Units | Method of analysis | Batch number | |||||
|---|---|---|---|---|---|---|---|---|
| #1 | #2 | #3 | #4 | #5 | #6 | |||
| Species identity L. minor (L) W. globosa (W) | % | Morphological analysis | 75 (L) 25 (W) | 82.3 (L) 17.7 (W) | 74.4 (L) 25.6 (W) | 89 (L) 11 (W) | 87 (L) 13 (W) | |
| Chemical parameters | ||||||||
| Moisture | % | AOCS Ba 2a‐38 | 2.47 | 5.61 | 2.86 | 5.51 | 1.60 | 2.33 |
| Crude protein | % | AOAC 990.03 | 46.17 | 41.93 | 43.13 | 41.30 | 45.26 | 42.92 |
| Crude fat | % | AOAC 922.06 | 9.28 | 7.70 | 9.09 | 9.12 | 9.71 | 8.91 |
| Ash | % | AOAC 923.03/32.1.05 16th Ed. | 5.03 | 4.95 | 5.11 | 4.52 | 4.34 | 4.11 |
| Carbohydrates | % | By differencea | NA | 3.9 | 3.4 | 3.5 | 5.7 | 10.5 |
| Dietary fibre (total) | % | AOAC 991.43 | 34.2 | 35.9 | 36.4 | 36.1 | 33.4 | 31.2 |
| Soluble | % | AOAC 991.43 | 0.7 | 4.5 | 2.5 | 3.5 | 2.3 | 5.6 |
| Insoluble | % | AOAC 991.43 | 33.5 | 31.4 | 33.9 | 32.6 | 31.1 | 25.6 |
| Minerals (micronutrients, trace elements and heavy metals) | ||||||||
| Potassium | mg/kg | AOAC 993.14 Mod. | 4,950 | 3,670 | 2,470 | 3,160 | 4,860 | 3,820 |
| Sodium | mg/kg | AOAC 993.14 Mod. | 1,680 | 2,320 | 1,200 | 1,340 | 1,660 | 1,340 |
| Magnesium | mg/kg | AOAC 993.14 Mod. | 3,430 | 3,460 | 3,890 | 3,430 | 3,200 | 3,090 |
| Aluminium | mg/kg | AOAC 993.14 Mod. | 3.2 | < 4.0 | 3.9 | 3.0 | 3.1 | 2.0 |
| Arsenic | mg/kg | AOAC 993.14 Mod. | 0.047 | 0.08 | 0.044 | 0.037 | 0.038 | 0.041 |
| Boron | mg/kg | AOAC 993.14 Mod. | 1,080 | 1,000 | 1,110 | 947 | 663 | 779 |
| Cadmium | mg/kg | AOAC 993.14 Mod. | 0.011 | < 0.01 | 0.014 | 0.01 | 0.006 | 0.006 |
| Calcium | mg/kg | AOAC 993.14 Mod. | 13,300 | 13,600 | 17,800 | 15,600 | 11,500 | 13,200 |
| Chromium | mg/kg | AOAC 993.14 Mod. | 0.334 | < 0.200 | 0.888 | 0.118 | < 0.200 | 0.177 |
| Cobalt | mg/kg | AOAC 993.14 Mod. | < 0.050 | < 0.050 | < 0.050 | < 0.050 | < 0.050 | < 0.050 |
| Copper | mg/kg | AOAC 993.14 Mod. | 2.27 | 2.74 | 2.79 | 1.95 | 3.63 | 2.88 |
| Iron | mg/kg | AOAC 993.14 Mod. | 358 | 301 | 348 | 237 | 525 | 361 |
| Lead | mg/kg | AOAC 993.14 Mod. | 0.011 | < 0.02 | 0.014 | 0.009 | 0.016 | 0.01 |
| Manganese | mg/kg | AOAC 993.14 Mod. | 333 | 128 | 263 | 157 | 322 | 223 |
| Mercury | mg/kg | AOAC 993.14 Mod. | < 0.015 | < 0.01 | < 0.015 | < 0.015 | < 0.015 | < 0.015 |
| Molybdenum | mg/kg | AOAC 993.14 Mod. | 0.100 | 0.104 | 0.076 | 0.066 | 0.122 | 0.071 |
| Phosphorus | mg/kg | AOAC 993.14 mod | 5780 | 4660 | 5810 | 4670 | 5120 | 4730 |
| Selenium | mg/kg | AOAC 993.14 mod | < 0.30 | 0.17 | < 0.30 | < 0.30 | < 0.30 | < 0.30 |
| Tin | mg/kg | AOAC 993.14 mod | < 0.15 | NA | < 0.15 | < 0.15 | < 0.15 | < 0.15 |
| Zinc | mg/kg | AOAC 993.14 mod | 56.6 | 40.2 | 71.1 | 57 | 100 | 76.4 |
| Antinutrients | ||||||||
| Oxalic Acid | % | AOAC 986.13 mod | 0.037 | 0.057 | 0.032 | NA | NA | 0.026 |
| Microbiological parameters | ||||||||
| Aerobic plate count | CFU/g | AOAC 990.12 | 420,000 | 58,000 | 54,000 | 47,000 | 26,000 | 120,000 |
| Salmonella spp. | Presence/Absence per g | AOAC 2003.09 | ND | ND | ND | ND | ND | ND |
| E. coli | MPN/g | FDA BAM Chapter 4 | > 1,100 | < 3 (method: AOAC 988.19) | < 3 | < 3 | < 3 | < 3 |
| Listeria spp. | Presence/Absence in 25 g | AOAC‐RI 050903 | ND | ND | ND | ND | ND | ND |
| Clostridium perfringens | CFU/g | AOAC 976.30 | < 10 | < 10 | < 10 | < 10 | < 10 | < 10 |
| Coliforms | CFU/g | AOAC 991.14 | < 10 | 100 | < 10 | < 10 | < 10 | < 10 |
| Yeasts | CFU/g | FDA BAM Chapter 18 | 4,500 | < 10 | 10 | 40 | < 10 | 20 |
| Moulds | CFU/g | FDA BAM Chapter 18 | < 10 | < 10 | 10 | 10 | < 10 | < 10 |
AOCS: American Oil Chemists’ Society; AOAC: Association of Official Analytical Collaboration; FDA: Food and Drug Administration; BAM: Bacteriological Analytical Manual; NA: Not Available; ND: Not Detected; CFU: colony forming units.
a
The applicant calculated carbohydrates as 100% – [protein % + moisture % + fat (AH) % + ash % + dietary fibre %], differently from Regulation (EU) No 1169/2011, in which carbohydrates include fibre.