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. 2021 Nov 1;12:775083. doi: 10.3389/fmicb.2021.775083

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Copyright © 2021 Fan, Luo, Zhang, Wang, Chen, Liu, Sun, Nan, Hiscox, Zhao and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Positive correlation between expression level and activity of CDC42 and naked HEV-3 infection in HepG2/C3A cells. (A) Relative CDC42 mRNA levels in HEV-infected HepG2/C3A cells with different pretreatment. Increasing of HEV-3 infection in the CDC42-overexpressed HepG2/C3A cells by IFA (B) and qPCR detection (C). IFA (D) and qPCR (E) detect non-enveloped HEV-3 in ML141-pretreated HepG2/C3A cells. The result of the qPCR assay was normalized to human GAPDH. ns, no significant; *, P < 0.05; ** and aa, P < 0.01; *** and aaa, P < 0.001.