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. 2021 Nov 1;12:775083. doi: 10.3389/fmicb.2021.775083

TABLE 1.

Primers used in gene expression studies.

Primers name Sequence (5′–3′)
aCDC42-F GAATTCATGCAGACGATTAA
aCDC42-R CTCGAGTAGCAGCACACACC
lenti-aCDC42-F GAATTCGCCGCCACCATGCAGACGATTAAGTGTGT
pLVX-aCDC42-R CTCGAGTCACTTATCGTCGTCATCCTTGTAATCTA GCAGCACACACCTGCGA
CDC42-qF ATTAAGTGYGTWGTTGTGGGY
CDC42-qR TCATMACTGTKACWGCATAGTT
GAPDH-gallus-qF AAACTCATTGTCATACCAGG
GAPDH-gallus-qR ATACACAGAGGACCAGGTTG
CaHEV-Taqman-F TATGTGCTGCGGGGTGTCAA
CaHEV-Taqman-R CATCTGGTACCGTGCGAGTA
Probe-ORF3-CaHEV FAM-CTCCCAAACGCTCCCAGCCGGA-BHQ
EF1 ATGTTGGTGGGGTGCTGGTCGAGATTG
ER1 GGGTTGATTGGTCCGATATGATGCCAG
EF2 TTGTTGGACATACCCCCGGCCCACA
ER2 TAATCACCGCAAGACGGCTAGTGG
HEV-ORF1-qF GTTGAGCAGAACCCGAAGAG
HEV-ORF1-qR CGGGCTCAGTCAAGTAAAGC
HEV-ORF3-qF GGTGGTTTCTGGGGTGAC
HEV-ORF3-qR AGGGGTTGGTTGGATGAA
Probe-ORF3-HEV FAM-TGATTCTCAGCCCTTCGC-BHQ
GAPDH-qF ACAAGGCTGGGGCTCATTTG
GAPDH-qR AGGGGCCATCCACAGTCTTC