Table 1. The mean Ct is the mean of three technical replicates. Expected Ct assumes that halving starting concentration should increase the Ct value by 1.

copies/mL	mean Ct	expected Ct	expected Ct - mean Ct
100017	26.99	26.99	0
50008	27.95	27.99	0.04
33292	28.77	28.58	-0.19
24933	28.86	28.99	0.13
19975	28.92	29.31	0.39
12467	29.32	29.99	0.67
10058	29.83	30.3	0.47
4958	30.98	31.32	0.34
3400	31.42	31.87	0.45
2550	31.92	32.28	0.36
1983	32.21	32.65	0.44
1275	33.46	33.28	-0.18
1000	33.73	33.63	-0.1
500	34.21	34.63	0.42
333	35.6	35.22	-0.38
250	34.86	35.63	0.77