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. 2021 Oct 13;6:268. [Version 1] doi: 10.12688/wellcomeopenres.17226.1

Table 3. Estimated optimal pool size based on prevalence.

Prevalence
range
Number of
samples per pool
Number of
samples per
pooled plate
Average number of
positive samples that
will need retest per pool
Average number of
positive samples that
will need retest per plate
0.1-0.2% 20 1880 2-4 40-80
0.3-0.4% 15 1410 5-6 75-90
0.5-0.9% 10 940 5-9 50-90
1.0-1.4% 8 752 7-11 56-88
1.5-2.6% 6 564 9-15 54-90
2.7-3.8% 5 470 13-18 65-90
3.9-6.1% 4 376 15-23 60-92
6.2-10.9% 3 282 18-31 54-93
11-24.4% 2 188 21-46 42-92