Figure EV3. Depletion of phagocytic cells in the lymph node and liver does not enhance CHIKV dissemination.

WT C57BL/6 mice were i.v. injected with PBS‐loaded liposomes (PLL) or clodronate‐loaded liposomes (CLL) 42 h prior to virus inoculation and subcutaneously injected in the left‐rear footpad (FP) with PLL or CLL 24 h prior to virus inoculation as indicated. Mice were then inoculated s.c. with 10³ PFU of CHIKV in the left‐rear footpad, and tissues and serum were collected at 24 hpi. Infectious virus was quantified by FFA. Mean ± SEM. Data are pooled from two experiments, n = 8. Two‐way ANOVA with Bonferroni’s multiple comparison test, comparing all groups to IV PLL+ FP PLL group; **P < 0.01, ****P < 0.0001.