Figure 1. CRL4^DCAF1 exists in a dimer/tetramer equilibrium.

1. Predicted domain organization of the human DCAF1 (FL).
2. SDS–PAGE Coomassie gel of the neddylation reaction of CRL4^DCAF1 at time 0 h and the fully neddylated N8‐CRL4^DCAF1 after 2 h.
3. SEC‐MALS analysis of CRL4^DCAF1 and N8‐CRL4^DCAF1. The chromatogram displays Rayleigh ratio curves for CRL4^DCAF1 (blue) and N8‐CRL4^DCAF1 (red) together with the molar mass (MDa) of the main peaks calculated by MALS. The table summarizes the SEC‐MALS observed molecular weights in the main peaks, the calculated molecular weights, polydispersity values, and oligomeric states of the tested complexes.

Source data are available online for this figure.