Figure EV4. CRL4^DCAF1 tetramerization and CSN binding are mutually exclusive.

1. Fitting the crystal structure of CSN (PDB 4D10) (wheat) into CRL4^DCAF1 cryo‐EM map.
2. Strep pull‐down assay of untagged CSN (E76A) complex on strep‐tagged N8‐CRL4^DCAF1(FL) (lane1), CRL4^DCAF1(FL) (lane2), CRL4^DCAF1(ΔNTD) (lane3), and N8‐CRL4^DCAF1(ΔNTD) (lane 4) (n = 2).
3. A representative cryo‐EM micrograph for CRL4^DCAF1‐CSN complex.
4. Workflow of cryo‐EM classification and refinement.
5. Fourier shell correlation curve (FSC). The resolution values reported for all reconstructions are based on the gold‐standard Fourier shell correlation curve (FSC) at 0.143 criterion and all the related FSC curves are corrected for the effects of soft masks using high‐resolution noise substitution.

Source data are available online for this figure.