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. 2021 Oct 6;40(22):e107958. doi: 10.15252/embj.2021107958

Figure 8. Ice2 promotes ER membrane biogenesis through inhibition of Pah1.

Figure 8

  1. Lipidomic analysis of WT and ∆ice2 cells in which PAH1 was replaced with PAH1‐HA or pah1(7A)‐HA as indicated (SSY2841, SSY2842, SSY2970). Mean + s.e.m., n = 4 biological replicates. Asterisks indicate statistical significance compared with WT cells, as judged by a two‐tailed Student’s t‐test assuming equal variance. **P < 0.01.
  2. Quantification of peripheral ER structures in WT cells in which PAH1 was replaced by PAH1‐HA or pah1(7A)‐HA, without and with overexpression of ICE2 (SSY2841, 2842, 2843, 2844). Bars are the mean percentage of cell cortex covered by tubules (purple) or sheets (green), n = 3 biological replicates. Upper error bars are s.e.m. for the sum of tubules and sheets, and lower error bars are s.e.m. for sheets. Asterisks indicate statistical significance compared with the respective control cells not overexpressing ICE2, as judged by a two‐tailed Student’s t‐test assuming equal variance. *P < 0.05; **P < 0.01; n.s., not significant.
  3. Quantification of peripheral ER structures in WT cells in which PAH1 was replaced by PAH1‐HA or pah1(7A)‐HA (SSY2841, 2842), treated with 8 mM DTT for 1 h. Mean + s.e.m., n = 4 biological replicates. Asterisks indicate statistical significance compared with WT cells, as judged by a two‐tailed Student’s t‐test assuming equal variance. *P < 0.05; **P < 0.01.
  4. Quantification of WT and ∆ice2 cells with Rtn1‐mCherry puncta after treatment with 8 mM DTT for 1 h. Mean + s.e.m., n = 3 biological replicates. Asterisks indicate statistical significance compared with WT cells, as judged by a two‐tailed Student’s t‐test assuming equal variance. *P < 0.05; n.s., not significant.

Source data are available online for this figure.