Figure EV4. Status of signal transduction pathways in Vav1^ΔC‐transformed cells.

1. Western blot showing the levels of phosphorylated and total proteins in spleen extracts from control and tumor‐bearing recipient mice that had been transplanted with the indicated retrovirally transduced CD4⁺ T cells (top). Total levels of Akt, Erk1/2, and actin were used as loading controls. The red asterisk indicates a panel that has been generated using an independent filter but the same tissue lysates.
2. Abundance of indicated transcripts (bottom) in splenic cells from control (EGFP⁺), tumor‐bearing mice (expressing EGFP and Vav1^ΔC), and mice transplanted with cells transduced with the catalytically dead version of Vav1^ΔC (expressing EGFP and Vav1^ΔC+E201A). Values are given relative to the expression of each transcript in samples obtained from EGFP controls (which was given an arbitrary value of 1). n = 4 animals per class analyzed. a.u., arbitrary unit. Data represent the mean ± SEM. Statistical values obtained using the Student’s t are given relative to control EGFP⁺ cells.

Source data are available online for this figure.