Figure 5. Vav1^ΔC triggers a vast proliferative program in transformed T_FH cells.

• A
  Genes up‐ (red) and downregulated (blue) in the tumor cells from lymph nodes (Vav1^ΔC LN) and spleen (Vav1^ΔC SP) of recipient mice transplanted with Vav1^ΔC‐expressing CD4⁺ T cells. As comparative control, we used CD4⁺ T cells from spleen (C SP) and lymph nodes (C LN) of control recipient mice. Rows represent individual genes. Columns represent experimental replicas. Total number of genes is indicated on the left. Relative changes in abundance are shown in color gradients according to the color scale shown on the right.
• B
  GSEA showing enrichment of T_FH‐associated gene signatures in the transcriptome of Vav1^ΔC‐transformed cells. The expression profile of the top 25 leading‐edge genes in the transcriptome of CD4⁺ T cells from healthy control (C) and Vav1^ΔC‐tumor‐bearing mice (Vav1^ΔC) is shown. The normalized enrichment scores (NES) and false discovery rate values (FDR, using q values) are indicated inside the GSEA graph. Relative changes in abundance are shown in color gradients according to the color scale shown at the bottom. q‐val, q value.
• C
  Main functional categories encoded by the Vav1^ΔC‐dependent transcriptome. For all of them, P ≤ 0.001 (Fisher’s exact test).
• D–I
  GSEAs showing enrichment of indicated gene signatures (top) in the transcriptome of Vav1^ΔC‐transformed cells. In each case, we show the expression profile of the top 25 leading‐edge genes in healthy control (C) and Vav1^ΔC‐tumor‐bearing mice (Vav1^ΔC). NES and FDR values (FDR, using q values) are indicated inside each GSEA graph. Relative changes in abundance are shown in color gradients according to the same color scale shown in panel (I).
• J
  Dot plot of the Vav1^ΔC‐dependent gene signature fit score in the indicated experimental groups (bottom) that were retrieved from a previous work (Zang et al, 2017). Dots represent values from an individual sample. Bars represent the mean enrichment value ± SEM for the overall sample set. ***P ≤ 0.001 (according to Tukey's honest significance difference test).
• K
  Venn diagrams representing the number of up‐ (left) and downregulated (right) genes from the Vav1^ΔC‐dependent transcriptome shared with the indicated datasets.
• L
  Level of enrichment of indicated gene signatures that are either Vav1^ΔC‐specific (red) or shared with the different datasets (blue) used in panel (J).