Fig. 4.

Diazepam produced diverse effects on the microstructure of repetitive self-grooming in the ASD model and stress model.

A, Diazepam treatment did not change the self-grooming microstructure and only reduced stage 0 (unpaired t-test, KO + S group n = 6 mice, KO + D group n = 9 mice). B-D, The switching frequency was reduced in the KO mice treated with diazepam (unpaired t-test). E, Diazepam treatment reduced the stage 2 and stage 3 of the self-grooming microstructure in restraint mice compared to saline injection (unpaired t-test, Restraint + S group n = 8 mice, Restraint + D group n = 6 mice). F–H, The switching frequency was reduced in restraint mice treated with diazepam (unpaired t-test). Data are shown as the means ± SEM. *p < 0.05, **p < 0.01.