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. Author manuscript; available in PMC: 2021 Nov 15.
Published in final edited form as: Glia. 2021 Feb 27;69(9):2059–2076. doi: 10.1002/glia.23984

FIGURE 1.

FIGURE 1

Schematic depiction of the RCAS/tv-a glioma model. (a) DF-1 chicken fibroblasts are transfected with RCAS plasmids carrying a gene of interest for propagation in vitro. (b) Transfected DF-1 cells or the isolated RCAS virions are injected into transgenic tv-a rodent hosts where they selectively infect TV-A-positive cells, in some instances confined by cell -or tissue-specific tv-a gene expression. Stable integration of the gene of interest into host DNA then results in tumors with specific features. Abbreviations: RCAS, replication-competent ASLV long terminal repeat splice acceptor; tv-a, tumor virus receptor A