Figure 10.

Compound 20a inhibited the migration capacities of NCI-H460 cells. (A) Representative images of the wound healing assay carried out on NCI-H460 cells treated at 5 μΜ, 10 μM, and 20 μM. A significant open wound area was observed after the 24 h time point in both 10 and 20 μM treated NCI-H460 cells. The wound area was quantified by ImageJsoftware. Data is the representation of three independent experiments ± S. D. **p < 0.01, ***p < 0.001. (B) Graph representing a dose dependent antimigration effect of 20a in a time interval of 24 h. (C) Effect of compound 20a on the expression of Vimentin and VEGF. A suppression effect on the expression of VEGF was observed in a dose dependent manner (at 1 μM, 2 μM, 5 μM, 10 μM, and 20 μM), while no change in the expression of vimentin marker was observed.