TABLE 1.

k_cat and K_m values of ATP and S-1 peptide for WT and mutant EphA4_CYTO proteins^a

Protein	Km (mM)		kcat (dA340/mm/mM)
	ATP	S-1 peptide
WT	0.15 ± 0.01	0.01 ± 0.002	719 ± 46.6
YACTF	0.40 ± 0.07	2.00 ± 0.02	178.4 ± 14.8
YJX1F	0.17 ± 0.03	0.08 ± 0.03	302.9 ± 18.8
YJX2F	0.19 ± 0.01	0.51 ± 0.02	117.2 ± 41.4
YJX1+2F	0.14 ± 0.03	NA	NA

^aReactions were performed as described in the legend to Fig. 9. NA indicates values that were not available due to solubility (see text). Values are means of at least four determinations. Errors reported are standard deviations.