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. 2021 Nov 15;40:361. doi: 10.1186/s13046-021-02149-5

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© The Author(s) 2021, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Identification and characterization of a cisplatin sensitivity-associated circRNA (cDOPEY2). A The cisplatin sensitivity of two cisplatin-resistant ESCC cell lines (ECA109-CR and TE1-CR) was determined by a clonogenic formation assay. B A heatmap showing the top 5 most upregulated circRNAs and 5 most downregulated circRNAs in ESCC tissues and paired adjacent nontumorous tissues (PANTs) in GSE131969. cDOPEY2 (hsa_circ_0008078) is indicated by the red box. C qPCR analysis of the RNA levels of the indicated circRNAs with or without RNase R treatment. D qPCR analysis of the RNA levels of the indicated circRNAs in cisplatin-resistant cells and their corresponding parental cells. E A schematic diagram showing that cDOPEY2 was formed by the back-splicing of linear cDOPEY2 between the 20th and 24th exons. F The cDOPEY2 junction site was identified by Sanger sequencing. G qPCR analysis of cDOPEY2 and its linear counterpart DOPEY2 in complementary DNA (cDNA) and genomic DNA (gDNA). H Time-course qPCR analysis of cDOPEY2 and its linear counterpart DOPEY2 in ECA109 cells treated with actinomycin D (5 µg/mL). I qPCR amplification of cDOPEY2 and its linear counterpart DOPEY2 using random hexamer primers and oligo (dT) primers. J Subcellular qPCR analysis showing that cDOPEY2 was mainly localized in the cytoplasm. GAPDH and U1 were applied as positive controls in the cytoplasm and nucleus, respectively. K RNA fluorescence in situ hybridization analysis revealed the subcellular localization of cDOPEY2. Scale bar, 20 μm. L Relative expression of cDOPEY2 in a set of ESCC cell lines and normal esophageal epithelial cells (Het-1a and HEEC). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P values were determined using the unpaired Student’s t-test (A, C, D, H and I)