A&B Ca2+ transient particles heat-map images in ICC-MY under control conditions A and in the presence of NNC 55–0396 (10 μM) B. Active firing sites were color-coded and plotted as an occurrence maps in the ICC-MY network under control C and in the presence of NNC 55–0396 D. Trace plots of Ca2+ transient PTCLs activity of ICC-MY in control conditions showing PTCL area (blue) and PTCL count (green) E and in the presence of NNC 55–0396 F. Summary graphs of average percentage changes in PTCL area G, PTCL count H, the number of PTCL active sites I. J Average percentage changes of Ca2+ transient clusters (CTCs) duration. Data were normalized to controls and expressed as percentages (%). Significance determined using unpaired t-test, *** = P < 0.001, **** = P < 0.0001, n = 6. All data graphed as mean ± SEM.