Fig. 8. Effects of T-type Ca²⁺ channel antagonist, NNC 55–0396 on corpus ICC-MY Ca²⁺ transients.

A&B Ca²⁺ transient particles heat-map images in ICC-MY under control conditions A and in the presence of NNC 55–0396 (10 μM) B. Ca²⁺ activity is color-coded with warm areas (white, red) representing bright areas of Ca²⁺ fluorescence and cold colors (purple, black) representing dim areas of Ca²⁺ fluorescence. Scale bar is 30 mm in both A & B. Active firing sites were color-coded and plotted as an occurrence maps in the ICC-MY network under control C and in the presence of NNC 55–0396 D. Trace plots of Ca²⁺ transient PTCL activity of ICC-MY in control conditions showing PTCL area (blue) and PTCL count (green) E and in the presence of NNC 55–0396 F. Summary graphs of average percentage changes in Ca²⁺ PTCL area G, PTCL count H, the number of PTCL active sites I. J Average percentage changes of Ca²⁺ transient clusters (CTCs) duration. Data were normalized to controls and expressed as percentages (%). Significance determined using unpaired t-test, **** = P < 0.0001, n = 5. All data graphed as mean ± SEM.