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. 2021 Nov 10;14(11):dmm049077. doi: 10.1242/dmm.049077

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Surgical procedure and microinjection. (A) Surgical procedure for ultrasound-guided microinjection into the fetal left atrium. (B) Pre-injection B-mode image (top), microinjection needle advancement B-mode image (middle) and post-injection B-mode image (bottom) from a single representative fetus in the embolized group. In the top panel, the yellow square highlights the region showing the top and bottom walls of the left atrium as bright, horizontal lines. Following injection, a dark spot can be seen in between the top and bottom walls of the left atrium, confirming that embolization was successful (see yellow square in the bottom panel). B, blocker; M, microinjection glass needle; P, placenta; S, spine. Scale bars: 1 mm.