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UNC-86 and MEC-3–GAD (here shown as MEC-3AD) interact in a yeast in vivo system. (A) Schematic representation of the yeast system. UNC-86 and MEC-3–GAD interact in the presence of appropriate DNA binding sites. (B) Four transformants of RB101, each expressing either UNC-86 (row 1) or MEC-3–GAD (row 2) alone or both proteins (row 3) were dotted on X-Gal plates, and color development was scored after 3 days at 30°C.
