TABLE 2.
Effects of amino acid substitutions in UNC-86 on lacZ reporter gene activation in yeast strain RB101
| UNC-86 variant | Relative activity (%)a | Relative activity with MEC-3–GAD (%)a | Fold increaseb |
|---|---|---|---|
| Wild type | 100 ± 14 | 4,860 ± 225 | 49 |
| E149Ac | 47.6 ± 9.5 | 1,950 ± 333 | 40 |
| D296Ac | 90.5 ± 42.9 | 4,670 ± 670 | 52 |
| Class I | |||
| R146Ac | 57.1 ± 9.5 | 885 ± 124 | 15 |
| L197A | 65.9 ± 5.5 | 247 ± 44 | 4 |
| M202L | 139 ± 15 | 474 ± 90 | 3 |
| K206E | 57.8 ± 6.8 | 517 ± 97 | 9 |
| R242G | 58.8 ± 4.1 | 983 ± 94 | 17 |
| F297S | 194 ± 14 | 603 ± 49 | 3 |
| Class II | |||
| I189V | 5.0 ± 0.6 | 538 ± 62 | 108 |
| K250D | 2.2 ± 0.3 | 92 ± 37 | 42 |
| L253P | 6.2 ± 0.6 | 233 ± 24 | 38 |
| Q255P | 14.6 ± 1.6 | 578 ± 43 | 40 |
| F257L | 8.2 ± 1.9 | 605 ± 63 | 74 |
| Q259P | 10.2 ± 0.4 | 279 ± 67 | 27 |
| Class III | |||
| K156E | 7.4 ± 1.3 | 7.7 ± 1.5 | 1 |
| Q166R | 4.3 ± 0.4 | 4.8 ± 0.6 | 1 |
| T188A | 8.4 ± 2.3 | 11.6 ± 2.2 | 1 |
| R295Ac | 18.1 ± 11.9 | 3.8 ± 1.0 | 0 |
| I189T R242G | 0.01 ± 0.01 | 0.01 ± 0.01 | 1 |
a
Activity is shown as the mean ± the standard error relative to the activity of wild-type UNC-86, which was set at 100%.
b
Values represent the ratio of the mean relative activity with MEC-3–GAD and the mean relative activity of the respective UNC-86 variant alone.
c
Analyzed in an alanine substitution screen.